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n terminal sv40 nls sequence present  (Addgene inc)


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    Structured Review

    Addgene inc n terminal sv40 nls sequence present
    N Terminal Sv40 Nls Sequence Present, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/n+terminal+sv40+nls+sequence+present/pAAV-mDlx-NLS-mRuby2+(Plasmid+%2399130)/pm31725765-125-6-13
    Average 95 stars, based on 24 article reviews
    n terminal sv40 nls sequence present - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Synthesized:

    Article Title: Delivering genes across the blood-brain barrier: LY6A, a novel cellular receptor for AAV-PHP.B capsids
    Article Snippet: GFP, 2A-luciferase, Ly6a and Ly6c1 (splice variant 1) cDNAs were synthesized as gBlocks (IDT). .. The NLS-GFP were synthesized using the N-terminal SV40 NLS sequence present in the Addgene plasmid #99130. .. The CMV-SaCAS9 vector (AAV-CMV∷NLS-SaCas9-NLS-3xHA-bGHpA;U6∷BsaI-sgRNA) was obtained from Dr. Feng Zhang through Addgene (#61591). sgRNAs specifically targeting Ly6a or Ly6c1 were cloned after the U6 promoter using a single bridge oligo for each reaction as recommended (HiFi DNA Assembly, NEB).

    Article Title: Delivering genes across the blood-brain barrier: LY6A, a novel cellular receptor for AAV-PHP.B capsids.
    Article Snippet: GFP, 2A-luciferase, Ly6a, and Ly6c1 (splice variant 1) cDNAs were synthesized as gBlocks (IDT). .. The NLS-GFP were synthesized using the N-terminal SV40 NLS sequence present in the Addgene plasmid #99130. .. The CMV-SaCAS9 vector (AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA) was obtained from Dr. Feng Zhang through Addgene (#61591). sgRNAs specifically targeting Ly6a or Ly6c1 were cloned after the U6 promoter using a single bridge oligo for each reaction as recommended (HiFi DNA Assembly, NEB).

    Article Title: Delivering genes across the blood-brain barrier: LY6A, a novel cellular receptor for AAV-PHP.B capsids
    Article Snippet: GFP, 2A-luciferase, Ly6a , and Ly6c1 (splice variant 1) cDNAs were synthesized as gBlocks (IDT). .. The NLS-GFP were synthesized using the N-terminal SV40 NLS sequence present in the Addgene plasmid #99130. .. The CMV-SaCAS9 vector (AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA) was obtained from Dr. Feng Zhang through Addgene (#61591). sgRNAs specifically targeting Ly6a or Ly6c1 were cloned after the U6 promoter using a single bridge oligo for each reaction as recommended (HiFi DNA Assembly, NEB).

    Sequencing:

    Article Title: Delivering genes across the blood-brain barrier: LY6A, a novel cellular receptor for AAV-PHP.B capsids
    Article Snippet: GFP, 2A-luciferase, Ly6a and Ly6c1 (splice variant 1) cDNAs were synthesized as gBlocks (IDT). .. The NLS-GFP were synthesized using the N-terminal SV40 NLS sequence present in the Addgene plasmid #99130. .. The CMV-SaCAS9 vector (AAV-CMV∷NLS-SaCas9-NLS-3xHA-bGHpA;U6∷BsaI-sgRNA) was obtained from Dr. Feng Zhang through Addgene (#61591). sgRNAs specifically targeting Ly6a or Ly6c1 were cloned after the U6 promoter using a single bridge oligo for each reaction as recommended (HiFi DNA Assembly, NEB).

    Article Title: Delivering genes across the blood-brain barrier: LY6A, a novel cellular receptor for AAV-PHP.B capsids.
    Article Snippet: GFP, 2A-luciferase, Ly6a, and Ly6c1 (splice variant 1) cDNAs were synthesized as gBlocks (IDT). .. The NLS-GFP were synthesized using the N-terminal SV40 NLS sequence present in the Addgene plasmid #99130. .. The CMV-SaCAS9 vector (AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA) was obtained from Dr. Feng Zhang through Addgene (#61591). sgRNAs specifically targeting Ly6a or Ly6c1 were cloned after the U6 promoter using a single bridge oligo for each reaction as recommended (HiFi DNA Assembly, NEB).

    Article Title: Delivering genes across the blood-brain barrier: LY6A, a novel cellular receptor for AAV-PHP.B capsids
    Article Snippet: GFP, 2A-luciferase, Ly6a , and Ly6c1 (splice variant 1) cDNAs were synthesized as gBlocks (IDT). .. The NLS-GFP were synthesized using the N-terminal SV40 NLS sequence present in the Addgene plasmid #99130. .. The CMV-SaCAS9 vector (AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA) was obtained from Dr. Feng Zhang through Addgene (#61591). sgRNAs specifically targeting Ly6a or Ly6c1 were cloned after the U6 promoter using a single bridge oligo for each reaction as recommended (HiFi DNA Assembly, NEB).

    Plasmid Preparation:

    Article Title: Delivering genes across the blood-brain barrier: LY6A, a novel cellular receptor for AAV-PHP.B capsids
    Article Snippet: GFP, 2A-luciferase, Ly6a and Ly6c1 (splice variant 1) cDNAs were synthesized as gBlocks (IDT). .. The NLS-GFP were synthesized using the N-terminal SV40 NLS sequence present in the Addgene plasmid #99130. .. The CMV-SaCAS9 vector (AAV-CMV∷NLS-SaCas9-NLS-3xHA-bGHpA;U6∷BsaI-sgRNA) was obtained from Dr. Feng Zhang through Addgene (#61591). sgRNAs specifically targeting Ly6a or Ly6c1 were cloned after the U6 promoter using a single bridge oligo for each reaction as recommended (HiFi DNA Assembly, NEB).

    Article Title: Delivering genes across the blood-brain barrier: LY6A, a novel cellular receptor for AAV-PHP.B capsids.
    Article Snippet: GFP, 2A-luciferase, Ly6a, and Ly6c1 (splice variant 1) cDNAs were synthesized as gBlocks (IDT). .. The NLS-GFP were synthesized using the N-terminal SV40 NLS sequence present in the Addgene plasmid #99130. .. The CMV-SaCAS9 vector (AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA) was obtained from Dr. Feng Zhang through Addgene (#61591). sgRNAs specifically targeting Ly6a or Ly6c1 were cloned after the U6 promoter using a single bridge oligo for each reaction as recommended (HiFi DNA Assembly, NEB).

    Article Title: Delivering genes across the blood-brain barrier: LY6A, a novel cellular receptor for AAV-PHP.B capsids
    Article Snippet: GFP, 2A-luciferase, Ly6a , and Ly6c1 (splice variant 1) cDNAs were synthesized as gBlocks (IDT). .. The NLS-GFP were synthesized using the N-terminal SV40 NLS sequence present in the Addgene plasmid #99130. .. The CMV-SaCAS9 vector (AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA) was obtained from Dr. Feng Zhang through Addgene (#61591). sgRNAs specifically targeting Ly6a or Ly6c1 were cloned after the U6 promoter using a single bridge oligo for each reaction as recommended (HiFi DNA Assembly, NEB).



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    Addgene inc n terminal sv40 nls sequence present
    N Terminal Sv40 Nls Sequence Present, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/n+terminal+sv40+nls+sequence+present/pAAV-mDlx-NLS-mRuby2+(Plasmid+%2399130)/pm31725765-125-6-13
    Average 95 stars, based on 1 article reviews
    n terminal sv40 nls sequence present - by Bioz Stars, 2026-09
    95/100 stars
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